Principal Scientist, Cell Engineering

San Francisco, CA /
Biology /
Full time
Our mission is to enable pharmaceutical and biotechnology companies to bring more cell and gene therapies to patients, by dramatically increasing the efficiency of viral vector production and reducing the cost of manufacturing. 64x Bio was founded by world renowned scientists and experts in gene therapy from Harvard and the Wyss Institute. Our rapidly growing and cross disciplinary team consists of creative bioengineers and synthetic biologists working alongside data scientists to create cutting-edge solutions to problems in gene therapy. 


    • Identify and lead integration of new cell line engineering technologies and emerging practices to both drive innovation and develop improved workflows. 
    • Support resource management and project planning across the broader scientific team. Hire, onboard, and train group members and/or other contracted support.
    • Direct, manage, and provide technical direction in cell line engineering and associated methods to scientists working on various projects to reliably meet project deadlines. 
    • Troubleshoot and guide the troubleshooting of complex cell line engineering challenges in the cell engineering group, through the application of mammalian tissue culture as well and genome engineering expertise as well as industry best practices.
    • Collaborate regularly with other groups at 64x Bio such as Viral Vector Biology and Molecular Biology as well as other teams such as Data Science, on experimental design, interpretation of data, and rigorous tracking and recalibration of project timelines and goals. 
    • Design, execute and oversee experimental efforts for projects in the cell engineering group.
    • Communicate experimental and project progress to leadership.
    • Collaborate with the Operations team and other teams at 64x to secure experimental resources to complete experiments in the cell engineering group in a timely manner.
    • Influence and support 64x Bio’s overall direction as a company as well as its evolving needs and company culture, as needed.

About You

    • PhD in synthetic biology, molecular biology, bioengineering, cell biology or related field.
    • 8+ years of industry (preferred) experience engineering genomes and mammalian cell lines in a functional genomics context. 
    • Proven track record of leading successful teams and mentoring scientists to meet project goals and deadlines.
    • Excellent project management and organizational skills, with the ability to systematically oversee/advance multiple projects simultaneously.
    • Demonstrated ability to successfully communicate and collaborate with cross-functional and interdisciplinary teams.
    • Excellent communication skills, with the ability to effectively convey project plans and complex technical information to a wide range of internal teams and leadership.
    • Deep expertise and experience with functional genomics and pooled screening methodologies. 
    • Deep expertise and experience with assays, methods and accompanying data formats used in molecular biology and the engineering of mammalian systems (e.g., CHO, HEK293).
    • Deep expertise and experience applying next generation sequencing (NGS) technologies in a functional genomics context.
    • Ability to context-switch between big picture/project planning and deep technical discussions, in a flexible and adaptable manner.
    • Ability to work with teams in a dynamic and fast-paced environment.
    • Very strong interpersonal skills and ability to collaborate with a wide array of personalities, backgrounds, and work styles.
    • Detail oriented.
    • Team oriented.


    • Familiarity with laboratory automation.
    • Familiarity with data analysis platforms (e.g., Python, R).
    • Expertise and experience with bioproduction, cell banking, regulatory testing, and GMP principles.