Scientist/Senior Scientist, Protein Purification

Massachusetts
Protein
Full-time

About Dragonfly
Founded in 2015 by Dr. Tyler Jacks, head of the Koch Institute at MIT, Dr. David Raulet, one of the world's leading experts in Natural Killer (NK) cell biology, and Bill Haney, a longtime tech entrepreneur and investor, Dragonfly Therapeutics develops novel first-in-class therapeutics targeted at Natural Killer cells and other cells of the innate immune system. Our therapies are designed to counterbalance immune suppressive factors present in the tumor microenvironment and mobilize anti-cancer immune responses, and are expected to be potent as single agents as well as in treatment combinations with existing cancer immunotherapies – such as T cell treatments.  Dragonfly’s Scientific Advisory Board and founders are major figures in cancer biology and immunology, and launched Dragonfly to harness the power of the immune system to provide breakthrough cancer treatments for patients.
 
Scientist/Senior Scientist, Protein Purification
We are looking for a highly motivated and talented biochemist to join our Biologics team. The successful candidate will be responsible for developing, implementing and performing protein purification of a wide variety of bispecific and trispecific antibody formats. He/She will be a key player in providing comprehensive biochemical characterization and developability assessment of different therapeutic candidates. The successful candidate will work with Dragonfly’s talented team to discover and develop therapies that stimulate immune responses against solid and hematologic cancers.

Position Overview:
·         Develop scalable purification schemes for a wide variety of bispecific antibodies, antibody fragments, antibody-fusions and other formats
·         Perform screening and in depth characterization of different therapeutic candidates
·         Design and implement developability assessment assays using state of the art techniques of protein characterization
·         Train junior personnel
 
Basic Qualifications:
·         M.Sc. with 5-10 years or Ph.D. in Biochemistry or related discipline with 3+ years of experience in purification/characterization  in an industrial setting (biotech, Pharma or CRO)
·         Extensive hands-on knowledge of purification of scFV, mono- and bispecific antibodies, tagged and untagged  recombinant proteins
·         Proficiency in various techniques of protein purification including affinity, IEX, SEC, HIC, CHT, and RP chromatographies
·         Hands on experience with AKTA and Bio-Rad chromatography systems and ability to troubleshoot
·         Extensive experience with protein characterization techniques such as SDS-PAGE, Western blot, SEC-HPLC, DSF, RP-HPLC
·         Hands on experience with SPR (Biacore T200 or 8K) would be a plus
·         Strong interpersonal and communication skills, ability to collaborate and communicate across disciplines
·         Hands on experience with protein expression in ExpiCHO or Expi293 is a plus
·         Knowledge of immune-oncology space is desirable
 

We look forward to reviewing your application! Please give us 10 days to reply~

Best,
Dragonfly HR team