Research Associate, Protein Expression and Purification

Massachusetts
Research
Full-time

About Dragonfly
Founded in 2015 by Dr. Tyler Jacks, head of the Koch Institute at MIT, Dr. David Raulet, one of the world's leading experts in Natural Killer (NK) cell biology, and Bill Haney, a longtime tech entrepreneur and investor, Dragonfly Therapeutics develops novel first-in-class therapeutics targeted at Natural Killer cells and other cells of the innate immune system. Our therapies are designed to counterbalance immune suppressive factors present in the tumor microenvironment and mobilize anti-cancer immune responses, and are expected to be potent as single agents as well as in treatment combinations with existing cancer immunotherapies – such as T cell treatments.  Dragonfly’s Scientific Advisory Board and founders are major figures in cancer biology and immunology, and launched Dragonfly to harness the power of the immune system to provide breakthrough cancer treatments for patients.

Research Associate, Protein Expression and Purification
Dragonfly Therapeutics seeks an enthusiastic and motivated Research Associate to support new cancer drug development. The successful candidate will work with Dragonfly’s team to discover and develop novel therapies that stimulate immune responses against solid and hematologic cancers.
 
Responsibilities:
·         Recombinant protein expression in mammalian cells by transient transfections
·         Molecular biology: PCR, primer design, DNA cloning (Gibson), DNA sequence analysis (SnapGene), restriction digests, transformations, mini and maxi preps
·         Protein purification: affinity, size exclusion and ion exchange chromatography
·         Operation of purification chromatography equipment (GE Akta, BioRad NGC)
·         Protein characterization: SDS-PAGE, Western blot, Protein thermal shift, and ELISA
·         Aseptic mammalian cell culture
·         Generate, analyze, interpret, organize, and present experimental data
·         Follow SOPs, develop new assays and troubleshoot when needed
·         Manage daily work flow independently and seek advice when appropriate
·         Work collaboratively within the Dragonfly team
 
Qualifications:
·         B.S. or M.S. degree in biochemistry, chemical engineering, or relevant field
·         2+ years of relevant experience, industry experience preferred
·         Hands on experience with DNA cloning and molecular biology techniques
·         Experience in protein expression (transient and stable) and purification
·         Mastery of aseptic technique in cell culture
·         Knowledge of biochemistry, cell and molecular biology
·         Proficiency with data analysis methods and software
·         Strong organizational and record-keeping skills, and written and verbal communication skills
·         Ability to multi-task with a high attention to detail
·         Ability to work in concert with a team as well as independently
·         Self-motivated, conscientious, and enthusiastic about curing cancer