Scientist or Senior Scientist - Cas Nuclease Engineering

San Francisco
Genome Editing and Protein Discovery
Mammoth Biosciences is harnessing the diversity of nature to power the next generation of CRISPR products. Through the discovery and development of novel CRISPR products. Through the discovery and development of novel CRISPR systems, the company is enabling the full potential of its platform to read and write the code of life. Mammoth aims to democratize disease detection with an easy and affordable point-of-care test that allows real-time and simultaneous detection of multiple conditions. By leveraging its internal research and development and exclusive licensing to Cas12, Cas13 and Cas14, Mammoth can provide enhanced diagnostics and genome editing for life science research, healthcare, agriculture, biodefense and more. Based in San Francisco, Mammoth Biosciences is co-founded by CRISPR pioneer Jennifer Doudna and principal founders Trevor Martin, Janice Chen, and Lucas Harrington. The firm is backed by top institutional investors including Mayfield, NFX, and 8VC, Decheng and leading individual investors including Brook Byers, Tim Cook, and Jeff Huber.
Mammoth is seeking a Scientist or Sr Scientist with expertise in biomolecular engineering to join its growing Discovery team. The Discovery team is responsible for developing novel CRISPR-associated (Cas) nucleases for genome editing and diagnostic applications.
The successful candidate will be responsible for engineering CRISPR-associated (Cas) effector nucleases for altered and augmented functionality. The scope of the engineering work will include constructing and screening libraries for: (i) domain fusions with novel Cas nucleases, (ii) altered PAM specificity and (iii) optimized trans-cleavage activity. Over the first year of employment, the successful candidate will function as an individual contributor with efforts split 50-50 between execution and platform development. After the first year, the successful candidate will have the opportunity to re-shape their role to suit their interests.


    • Plan and execute engineering cycles for Cas effector nucleases
    • Work with the Director to set quarterly and annual goals for engineering work
    • Develop platform (assays, tools and infrastructure) to support engineering work
    • Document and regularly present experimental results at internal meetings
    • Train and Mentor Research Associates
    • Coordinate with Research Associates and computational biology staff to execute experiments


    • 3 years biomolecular engineering experience
    • PhD or 5 years experience in in the synthetic biology or biotechnology industry

Preferred Qualifications

    • Direct experience engineering Cas effector nucleases
    • Deep familiarity with CRISPR-Cas literature
    • Hands-on experience using E. coli as a host organism for biomolecular engineering or HTP screening
    • Hands-on experience using laboratory automation (liquid handlers and colony pickers) to execute experiments
    • Experience developing NGS assays
    • RNA biochemistry experience
    • Protein expression and purification experience
    • Computer and scripting skills (Benchling, Geneious, JMP, SQL, R, python)


    • Competitive salary with equity
    • Full health/vision/dental benefits
    • Unlimited vacation
    • Free breakfast/lunch/dinner/barista
    • Free gym
    • Public transportation commuter subsidy
    • 401(k) matching