How many years of hands-on experience do you have with human pluripotent stem cells (hESC or iPSC)? How many different lines have you maintained?

What assays did you do to check their quality?

We are not automating cell culture by fully replicating manual cell culture processes which often requires us to review the “why” of how things are done. Could you please tell us a time where you had to not follow manufacturer instructions to make something work?